Device

Part:BBa_K2008007:Design

Designed by: Rachelle Varga   Group: iGEM16_UofC_Calgary   (2016-10-09)


Xylose->comK with AmyE homology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 862


Design Notes

Two internal EcoRI restriction sites and one internal SpeI restriction site to allow for this part to be BioBrick compatible. Flanking amyE regions of homology were added to allow for chromosomal integration and increased long-term stability of the comK construct.


Source

This part was amplified from the pAX01-comK plasmid constructed by Zhang and Zhang, 2010, and was previously characterized by the UofC_Calgary 2014 iGEM team. Flanking amyE regions of homology were derived from the B. subtilis 168 genome.

References